ICUS Weekly News Monitor 2-12-2016

1.  Atherosclerosis,  July, 2015,  Therapeutic ultrasound: Increased HDL-Cholesterol following
infusions of acoustic microspheres and apolipoprotein A-I plasmids   
Authors: Jason W. Castle et al.
2.  Echo Research and Practice,  Feb 1, 2016,  Echo Research and Practice is now indexed in PubMed!
Atherosclerosis 241 (2015) 92e99
July, 2015
Therapeutic ultrasound: Increased HDL-Cholesterol following
infusions of acoustic microspheres and apolipoprotein A-I plasmids
Authors: Jason W. Castle a, *, Kevin P. Kent b, Ying Fan a, Kirk D. Wallace a, Cynthia E.L. Davis a,
Jeannette C. Roberts a, Michael E. Marino a, Kai E. Thomenius a, Hae W. Lim b, Eric Coles c,
Michael H. Davidson c, d, Steven B. Feinstein c, e, Anthony DeMaria f
a General Electric Global Research, Niskayuna, NY, USA
b Formerly GE Global Research, Niskayuna, NY, USA
c SonoGene LLC, Glen Ellyn, IL, USA
d University of Chicago, Pritzker School of Medicine, Chicago, IL, USA
e Rush University Medical Center, Chicago, IL, USA
f Sulpizio Cardiovascular Center, University of California, San Diego, CA, USA
Background: Low levels of HDL-C are an independent cardiovascular risk factor associated with increased premature cardiovascular death. However, HDL-C therapies historically have been limited by issues relating to immunogenicity, hepatotoxicity and scalability, and have been ineffective in clinical trials.
Objective: We examined the feasibility of using injectable acoustic microspheres to locally deliver human ApoA-I DNA plasmids in a pre-clinical model and quantify increased production of HDL-C in vivo.
Methods: Our novel site-specific gene delivery system was examined in naïve rat model and comprised the following steps: (1) intravenous co-administration of a solution containing acoustically active microspheres (Optison™, GE Healthcare, Princeton, New Jersey) and human ApoA-I plasmids; (2) ultrasound verification of the presence of the microspheres within the liver vasculature; (3) External application of locally-directed acoustic energy, (4) induction of microsphere disruption and in situ sonoporation; (4) ApoA-I plasmid hepatic uptake; (5) transcription and expression of human ApoA-I protein; and (6) elevation of serum HDL-C.
Results: Co-administration of ApoA-I plasmids and acoustic microspheres, activated by external ultrasound energy, resulted in transcription and production of human ApoA-I protein and elevated serum HDL-C in rats (up to 61%; p-value < 0.05).
Conclusions: HDL-C was increased in rats following ultrasound directed delivery of human ApoA-I plasmids by microsphere sonoporation. The present method provides a novel approach to promote ApoA-I synthesis and nascent HDL-C elevation, potentially permitting the use of a minimally-invasive ultrasound-based, gene delivery system for treating individuals with low HDL-C.
Echo Research and Practice
Feb 1, 2016
Echo Research and Practice is now indexed in PubMed!
Echo Research and Practice, the open-access journal endorsed  by the International Contrast Ultrasound Society (ICUS), is now indexed in PubMed. In addition, the journal has been selected for indexing in the Web of Science’s Emerging Sources Citation Index.  Authors submitting their work to the journal can expect to receive an initial response in an average of 25 days, and accepted work will be published in 2 months average.  Members of ICUS receive a 10% discount on the article publication charge.  Authors receive suggestions for increasing post-publication impact, the results of which are visible with article-level metrics. To submit your work in Echo Research and Practice go to:

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